BMRB Entry 11209

Title:
Solution structure of the SH3 domain of Endophilin B1 (Sh3g1b1)
Deposition date:
2010-07-22
Original release date:
2011-07-21
Authors:
Qin, X.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain of Endophilin B1 (Sh3g1b1)"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P041220-01

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts74
1H chemical shifts518

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain GRB2-like protein B11

Entities:

Entity 1, SH3 domain GRB2-like protein B1 81 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLEUASNASP
2   LEULYSGLUSERSERASNASNARGLYSALA
3   ARGVALLEUTYRASPTYRASPALAALAASN
4   SERTHRGLULEUSERLEULEUALAASPGLU
5   VALILETHRVALPHESERVALVALGLYMET
6   ASPSERASPTRPLEUMETGLYGLUARGGLY
7   ASNGLNLYSGLYLYSVALPROILETHRTYR
8   LEUGLULEULEUASNSERGLYPROSERSER
9   GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.52 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, DelaglioF. - processing

NMRView v5.0.4, JohnsonB.A. - data analysis

Kujira v0.9296, KobayashiN. - data analysis

CYANA v2.0.17, GuntertP. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAE34495
GB ABA54268 EDL12028
REF NP_001268971

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks