BMRB Entry 17889

Title:
The polyserine tract of Nasonia vitripennis Vg residues 351-385
Deposition date:
2011-08-29
Original release date:
2012-05-22
Authors:
Havukainen, Heli; Halskau, Oyvind
Citation:

Citation: Havukainen, Heli; Underhaug, Jarl; Wolschin, Florian; Amdam, Gro; Halskau, Oyvind. "A vitellogenin polyserine cleavage site: highly disordered conformation protected from proteolysis by phosphorylation."  J. Exp. Biol. 215, 1837-1846 (2012).
PubMed: 22573762

Assembly members:

Assembly members:
NvVg, polymer, 35 residues, 4046.233 Da.

Natural source:

Natural source:   Common Name: Jewel Wasp   Taxonomy ID: 7425   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Nasonia vitripennis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NvVg: EHKHSDESTSESFESIADNN DDSYFQRKPKLTEAP

Data sets:
Data typeCount
15N chemical shifts37
1H chemical shifts222

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NvVg1

Entities:

Entity 1, NvVg 35 residues - 4046.233 Da.

1   GLUHISLYSHISSERASPGLUSERTHRSER
2   GLUSERPHEGLUSERILEALAASPASNASN
3   ASPASPSERTYRPHEGLNARGLYSPROLYS
4   LEUTHRGLUALAPRO

Samples:

sample_1: NvVg, [U-15N]-ILE, 20 w/v; sodium phosphate 50 mM; sodium azide 0.02%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.06 M; pH: 6.7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

ARIA v1.2, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Bruker AV600 600 MHz

Related Database Links:

PDB
REF XP_001607388

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks