BMRB Entry 19028

Title:
Flexible anchoring of archaeal MBF1 on ribosomes suggests role as recruitment factor
Deposition date:
2013-02-12
Original release date:
2014-06-05
Authors:
Launay, Helene; Blombarch, Fabian; van des Oost, John; Christodoulou, John
Citation:

Citation: Launay, Helene; Blombarch, Fabian; van des Oost, John; Christodoulou, John. "Flexible anchoring of archeal MBF1 on ribosomes suggests role as recruitment factor."  Nucleic Acids Res. ., .-..

Assembly members:

Assembly members:
MBF1-C, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: crenarchaeotes   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pWUR557

Data sets:
Data typeCount
13C chemical shifts238
15N chemical shifts77
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MBF1-C1

Entities:

Entity 1, MBF1-C 106 residues - Formula weight is not available

1   GLUASNALAGLULEUGLUILEVALTHRASP
2   TYRTYRLYSILEILELYSTHRALAARGGLU
3   GLNLEUGLYILESERGLNGLNGLNLEUALA
4   GLNLYSLEULYSVALSERGLUASNILEVAL
5   LYSARGPHEGLUSERGLYLYSLEULYSPRO
6   THRILESERGLNALAARGGLNLEUGLULYS
7   ILELEUGLYILELYSLEUVALTHRPROLEU
8   GLUASNASNGLUGLUSERGLULYSGLUPHE
9   ASPASPTHRGLYLEUTHRLEUGLYASPVAL
10   VALASNILELYSGLUGLYLYSLYSLEUGLU
11   HISHISHISHISHISHIS

Samples:

sample_1: MBF1-C, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 50 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

  • Bruker AMX 700 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AAK40608 ACX91484 AKA73586 AKA76284 AKA78976
REF WP_009990544

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks