BMRB Entry 19674

Title:
Solution structure of the CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE10
Deposition date:
2013-12-16
Original release date:
2015-01-12
Authors:
Bobay, Benjamin; DiGennaro, Peter; Bird, David
Citation:

Citation: DiGennaro, Peter; Bobay, Benjamin; Mck Bird, David. "Inferring function of CLE peptides from high resolution tertiary structures"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
entity, polymer, 12 residues, 1302.495 Da.

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: Synthetic   Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: RLVPSGPNPLHN

Data sets:
Data typeCount
13C chemical shifts38
15N chemical shifts11
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE101

Entities:

Entity 1, CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE10 12 residues - 1302.495 Da.

1   ARGLEUVALPROSERGLYPROASNPROLEU
2   HISASN

Samples:

sample_1: entity 4 mg/mL; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 140 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - refinement, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks