BMRB Entry 30045

Title:
DIY G-Quadruplexes: Solution structure of d(GGGTTTGGGTTTTGGGAGGG) in sodium
Deposition date:
2016-03-27
Original release date:
2017-03-30
Authors:
Dvorkin, S.; Karsisiotis, A.; Webba da Silva, M.
Citation:

Citation: Dvorkin, Scarlett; Karsisiotis, Andreas; Webba da Silva, Mateus. "Encoding canonical DNA quadruplex structure."  Sci Adv 4, eaat3007-eaat3007 (2018).
PubMed: 30182059

Assembly members:

Assembly members:
DNA (5'-D(*GP*GP*GP*TP*TP*TP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*AP*GP*GP*G)-3'), polymer, 20 residues, 6348.070 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA (5'-D(*GP*GP*GP*TP*TP*TP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*AP*GP*GP*G)-3'): GGGTTTGGGTTTTGGGAGGG

Data sets:
Data typeCount
1H chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 20 residues - 6348.070 Da.

1   DGDGDGDTDTDTDGDGDGDT
2   DTDTDTDGDGDGDADGDGDG

Samples:

sample_1: DNA, none, 1 ± 1.0 mM; H2O 93%; D2O 7%

sample_2: DNA, none, 1 ± 1 mM; D2O 100%

sample_conditions_1: ionic strength: 20 mM; pH: 6.8 pD; pressure: 1 atm; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
H,H jr-NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

Felix, Accelrys Software Inc. - chemical shift assignment, refinement

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

  • Varian VNMRS 500 MHz