BMRB Entry 30300

Title:
Solution NMR structure of human Rev1 (932-1039) in complex with ubiquitin
Deposition date:
2017-05-29
Original release date:
2018-05-17
Authors:
Cui, G.; Botuyan, M.; Mer, G.
Citation:

Citation: Cui, G.; Botuyan, M.; Mer, G.. "Solution NMR structure of human Rev1 (932-1039) in complex with ubiquitin"  J. Mol. Biol. 430, 2042-2050 (2018).
PubMed: 29778604

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, 8576.831 Da.
entity_2, polymer, 111 residues, 11985.354 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSUb

Data sets:
Data typeCount
13C chemical shifts802
15N chemical shifts173
1H chemical shifts1289

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 76 residues - 8576.831 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Entity 2, entity_2 111 residues - 11985.354 Da.

1   GLYHISMETPROSERPROSERGLNLEUASP
2   GLNSERVALLEUGLUALALEUPROPROASP
3   LEUARGGLUGLNVALGLUGLNVALCYSALA
4   VALGLNGLNALAGLUSERHISGLYASPLYS
5   LYSLYSGLUPROVALASNGLYCYSASNTHR
6   GLYILELEUPROGLNPROVALGLYTHRVAL
7   LEULEUGLNILEPROGLUPROGLNGLUSER
8   ASNSERASPALAGLYILEASNLEUILEALA
9   LEUPROALAPHESERGLNVALASPPROGLU
10   VALPHEALAALALEUPROALAGLULEUGLN
11   ARGGLULEULYSALAALATYRASPGLNARG
12   GLN

Samples:

sample_1: Sodium phosphate buffer 20 mM; Ubiquitin, [U-100% 13C; U-100% 15N], 1 mM

sample_2: Rev1, [U-100% 13C; U-100% 15N], 1 mM; Sodium phosphate buffer 50 mM; Ubiquitin 3 mM

sample_3: Rev1 3 mM; Sodium phosphate buffer 50 mM; Ubiquitin, [U-100% 13C; U-100% 15N], 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_2isotropicsample_conditions_2
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2
3D CBCA(CO)NHsample_2isotropicsample_conditions_2
3D HNCOsample_2isotropicsample_conditions_2
3D HN(CA)COsample_2isotropicsample_conditions_2
3D HBHA(CO)NHsample_2isotropicsample_conditions_2
3D H(CCO)NHsample_2isotropicsample_conditions_2
3D (H)CC(CO)NHsample_2isotropicsample_conditions_2
3D HCCH-TOCSYsample_2isotropicsample_conditions_2
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_2
3D 1H-15N NOESYsample_2isotropicsample_conditions_2
3D 1H-13C NOESYsample_2isotropicsample_conditions_2
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_2
13C,15N-filetered/edited NOESYsample_2isotropicsample_conditions_2
2D 1H-15N HSQCsample_3isotropicsample_conditions_2
2D 1H-13C HSQCsample_3isotropicsample_conditions_2
3D 1H-15N NOESYsample_3isotropicsample_conditions_2
3D 1H-13C NOESYsample_3isotropicsample_conditions_2
13C,15N-filetered/edited NOESYsample_3isotropicsample_conditions_2

Software:

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

SANE, Duggan, Legge, Dyson & Wright - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AvanceIII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks