BMRB Entry 30757

Title:
Structure of the C-terminal domain of BCL-XL in membrane
Deposition date:
2020-05-30
Original release date:
2020-06-26
Authors:
Yao, Y.; Tian, Y.; Marassi, F.
Citation:

Citation: Ryzhov, Pavel; Tian, Ye; Yao, Yong; Bobkov, Andrey; Im, Wonpil; Marassi, Francesca. "Conformational States of the Cytoprotective Protein Bcl-xL"  Biophys. J. 119, 1324-1334 (2020).
PubMed: 32888404

Assembly members:

Assembly members:
entity_1, polymer, 28 residues, 3174.721 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GQERFNRWFLTGMTVAGVVL LGSLFSRK

Data sets:
Data typeCount
13C chemical shifts43
15N chemical shifts23
1H chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 28 residues - 3174.721 Da.

1   GLYGLNGLUARGPHEASNARGTRPPHELEU
2   THRGLYMETTHRVALALAGLYVALVALLEU
3   LEUGLYSERLEUPHESERARGLYS

Samples:

sample_1: C-terminal domain of BCL-XL, [U-99% 15N], 0.2 mM

sample_2: C-terminal domain of BCL-XL, [U-99% 13C; U-99% 15N], 0.2 mM

sample_3: C-terminal domain of BCL-XL, [selectively 15N Leu labeled], 4 mg/mL

sample_4: C-terminal domain of BCL-XL, [selectively 15N Leu labeled], 10 mg/mL

sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 bar; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
2D SLFsample_3anisotropicsample_conditions_1
2D SLFsample_4anisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 500 MHz
  • Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks