BMRB Entry 34588

Title:
deoxyxylose nucleic acid hairpin
Deposition date:
2021-01-05
Original release date:
2021-02-05
Authors:
Mattelaer, C.; Mohitosh, M.; Smets, L.; Maiti, M.; Schepers, G.; Mattelaer, H.; Rosemeyer, H.; Herdewijn, P.; Lescrinier, E.
Citation:

Citation: Mattelaer, C.; Maiti, M.; Smets, L.; Maiti, M.; Schepers, G.; Mattelaer, H.; Rosemeyer, H.; Herdewijn, P.; Lescrinier, E.. "Stable hairpin structures formed by xylose based nucleic acids."  Chembiochem 22, 1638-1645 (2021).
PubMed: 33427360

Assembly members:

Assembly members:
entity_1, polymer, 20 residues, 6054.919 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XXXXXXXXXXXXXXXXXXXX

Data sets:
Data typeCount
13C chemical shifts66
1H chemical shifts167

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 20 residues - 6054.919 Da.

1   XAXGXCXAXAXTXCXCXCXC
2   XCXCXGXGXAXTXTXGXCXT

Samples:

sample_1: oligomer 1.9 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.9 pD; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker AVANCE II 600 MHz