BMRB Entry 5704

Title:
1H, 13C, and 15N assignment of mth677 protein from Methanobacterium Thermoautotrophicum
Deposition date:
2003-02-17
Original release date:
2003-06-09
Authors:
Blanco, Francisco; Campos-Olivas, Ramon; Yee, Adelinda; Arrowsmith, Cheryl; Rico, Manuel
Citation:

Citation: Blanco, Francisco; Campos-Olivas, Ramon; Yee, Adelinda; Arrowsmith, Cheryl; Rico, Manuel. "1H, 15N and 13C resonance assignment of the hypothetical protein mth677from Mthanobacterium thermoautotrophicum"  .

Assembly members:

Assembly members:
mth677, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Methanobacterium thermoautotrophicum   Taxonomy ID: 187420   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanothermobacter thermoautotrophicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts414
15N chemical shifts93
1H chemical shifts617

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mth6771

Entities:

Entity 1, mth677 94 residues - Formula weight is not available

1   GLYSERHISMETSERLEUARGLYSLEUTHR
2   GLUGLYASPLEUASPGLUILESERSERPHE
3   LEUHISASNTHRILESERASPPHEILELEU
4   LYSARGVALSERALALYSGLUILEVALASP
5   ILEASPILETHRVALLEUVALGLUTYRTHR
6   ASPGLULEULYSVALASPILESERALAGLU
7   LEUTYRLEUASPGLULEUSERASPALAASP
8   PROGLYILEVALASPGLUALAVALASPALA
9   ALATYRARGSERLEUGLUSERPHELEUASP
10   GLYPHEARGGLU

Samples:

sample_1: mth677, [U-15N], 1.2 mM; Sodium phosphate 25 mM; Sodim chloride 450 mM; DTT 10 mM; benzamidine 1 mM; H2O 90 % v/v; D2O 10 % v/v; Zinc chloride 20 uM

sample_2: mth677, [U-15N; U-13C], 1 mM; Sodium phosphate 25 mM; Sodim chloride 450 mM; DTT 10 mM; benzamidine 1 mM; H2O 90 % v/v; D2O 10 % v/v; Zinc chloride 20 uM

sample_3: mth677, [U-15N; U-13C], 1 mM; Sodium phosphate 25 mM; Sodim chloride 450 mM; DTT 10 mM; benzamidine 1 mM; D2O 100 % v/v; Zinc chloride 20 uM

condition_1: pH*: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

xwinnmr v3.1, Bruker - acquisition, plotting, processing

NMRPipe v97.027.12.56, . - data processing

NMRDraw v2.2, . - processing and analysis, spectral visualization

NMRView v5.0.3, . - spectra analysis, spectra visualization

CYANA v1.0.5, . - structure calculation

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAM69854
GB AAB85182
REF NP_275819 WP_010876315
SP O26773
AlphaFold O26773

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks