BMRB Entry 6392

Title:
Refined solution structure of the human TSG-6 Link module
Deposition date:
2004-11-17
Original release date:
2005-11-14
Authors:
Blundell, C.; Teriete, P.; Kahmann, J.; Pickford, A.; Campbell, I.
Citation:

Citation: Blundell, C.; Almond, A.; Mahoney, D.; Deangelis, P.; Campbell, I.; Day, A.. "Towards a Structure for a TSG-6{middle dot}Hyaluronan Complex by Modeling and NMR Spectroscopy: INSIGHTS INTO OTHER MEMBERS OF THE LINK MODULE SUPERFAMILY."  J. Biol. Chem. 280, 18189-18201 (2005).
PubMed: 15718240

Assembly members:

Assembly members:
human TSG-6, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
1H chemical shifts669
13C chemical shifts412
15N chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Link TSG61

Entities:

Entity 1, Link TSG6 98 residues - Formula weight is not available

1   GLYVALTYRHISARGGLUALAARGSERGLY
2   LYSTYRLYSLEUTHRTYRALAGLUALALYS
3   ALAVALCYSGLUPHEGLUGLYGLYHISLEU
4   ALATHRTYRLYSGLNLEUGLUALAALAARG
5   LYSILEGLYPHEHISVALCYSALAALAGLY
6   TRPMETALALYSGLYARGVALGLYTYRPRO
7   ILEVALLYSPROGLYPROASNCYSGLYPHE
8   GLYLYSTHRGLYILEILEASPTYRGLYILE
9   ARGLEUASNARGSERGLUARGTRPASPALA
10   TYRCYSTYRASNPROHISALALYS

Samples:

sample_1: human TSG-6 1.0 mM; Na ions 2 mM; D2O 100%

sample_2: human TSG-6, [U-15N], 2.0 mM; Na ions 2 mM

sample_3: human TSG-6, [U-15N; U-13C], 2.3 mM; Na ions 2 mM

sample_cond_1: pH: 6.0; temperature: 298 K; ionic strength: 2 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
15N-NOESY-HSQCnot availablenot availablenot available
15N-HSQC-NOESY-HSQCnot availablenot availablenot available
13C-NOESY-HSQCnot availablenot availablenot available
2D-NOESYnot availablenot availablenot available

Software:

FELIX v2.3 - spectrum processing

XEasy - spectrum analysis

NMR spectrometers:

  • n/a n/a 750 MHz
  • n/a n/a 750 MHz

Related Database Links:

BMRB 6393 7221 7222
PDB
DBJ BAE24204 BAJ20799
EMBL CAD12353 CAD13434 CAH74219
GB AAA03342 AAB00792 AAC53527 AAH21155 AAH30205
REF NP_001007814 NP_001075375 NP_001075780 NP_001252869 NP_009046
SP O08859 P98065 P98066
TPG DAA32671
AlphaFold O08859 P98065 P98066

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks