BMRB Entry 7078

Title:
1H Chemical Shift Assigments for a Potent Bowman-Birk Inhibitor from Lentil seeds
Deposition date:
2006-04-19
Original release date:
2006-09-08
Authors:
Ragg, Enzio; Galbusera, Valerio; Scarafoni, Alessio; Negri, Armando; Tedeschi, Gabriella; Consonni, Alessandro; Sessa, Fabio; Duranti, Marcello
Citation:

Citation: Ragg, Enzio; Galbusera, Valerio; Scarafoni, Alessio; Negri, Armando; Tedeschi, Gabriella; Consonni, Alessandro; Sessa, Fabio; Duranti, Marcello. "Inhibitory Properties and Solution Structure of a Potent Bowman-Birk Protease Inhibitor from Lentil (Lens culinaris, L.) Seeds"  FEBS J. 273, 4024-4039 (2006).
PubMed: 16889634

Assembly members:

Assembly members:
Bowman-Birk Inhibitor, polymer, 67 residues, 7448 Da.

Natural source:

Natural source:   Common Name: lentil   Taxonomy ID: 3864   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Lens culinaris

Experimental source:

Experimental source:   Production method: Purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Bowman-Birk Inhibitor: GDDVKSACCDTCLCTRSQPP TCRCVDVRESCHSACDKCVC AYSNPPQCQCYDTHKFCYKA CHNSEIE

Data sets:
Data typeCount
1H chemical shifts417

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LCTI monomer1

Entities:

Entity 1, LCTI monomer 67 residues - 7448 Da.

1   GLYASPASPVALLYSSERALACYSCYSASP
2   THRCYSLEUCYSTHRARGSERGLNPROPRO
3   THRCYSARGCYSVALASPVALARGGLUSER
4   CYSHISSERALACYSASPLYSCYSVALCYS
5   ALATYRSERASNPROPROGLNCYSGLNCYS
6   TYRASPTHRHISLYSPHECYSTYRLYSALA
7   CYSHISASNSERGLUILEGLU

Samples:

sample_1: Bowman-Birk Inhibitor 1 ± 0.1 mM; D2O 10%; H2O 90%

sample_2: Bowman-Birk Inhibitor 1 ± 0.1 mM; D2O 100%

conditions_1: ionic strength: 3 mM; pH: 3.1; temperature: 298 K

conditions_2: ionic strength: 3.0 mM; pH*: 3.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availableconditions_1
TOCSYsample_1not availableconditions_1
COSY-DQFsample_1not availableconditions_2

Software:

xwinnmr v2.6, Bruker Spectrospin - acquisition and processing

SPARKY v3.106, UCSF - assignment and cross-peak integration

NMR spectrometers:

  • Bruker Spectrospin AMX-600 600 MHz

Related Database Links:

PDB
EMBL CAR47883
GB AFN85540
SP Q8W4Y8
AlphaFold Q8W4Y8