BMRB Entry 7108

Title:
1H, 13C and 15N chemical shift assignments for TrxA (reduced form) from Bacillus subtilis
Deposition date:
2006-05-12
Original release date:
2008-06-23
Authors:
Xu, Huimin; Jin, Changwen
Citation:

Citation: Li, Y.; Hu, Y.; Zhang, X.; Xu, H.; Lescop, E.; Xia, B.; Jin, C.. "Conformational fluctuations coupled to the thiol-disulfide transfer between thioredoxin and arsenate reductase in Bacillus subtilis"  J. Biol. Chem. 282, 11078-11083 (2007).
PubMed: 17303556

Assembly members:

Assembly members:
TrxA, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts439
15N chemical shifts105
1H chemical shifts695

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TrxA monomer1

Entities:

Entity 1, TrxA monomer 104 residues - Formula weight is not available

1   METALAILEVALLYSALATHRASPGLNSER
2   PHESERALAGLUTHRSERGLUGLYVALVAL
3   LEUALAASPPHETRPALAPROTRPCYSGLY
4   PROCYSLYSMETILEALAPROVALLEUGLU
5   GLULEUASPGLNGLUMETGLYASPLYSLEU
6   LYSILEVALLYSILEASPVALASPGLUASN
7   GLNGLUTHRALAGLYLYSTYRGLYVALMET
8   SERILEPROTHRLEULEUVALLEULYSASP
9   GLYGLUVALVALGLUTHRSERVALGLYPHE
10   LYSPROLYSGLUALALEUGLNGLULEUVAL
11   ASNLYSHISLEU

Samples:

sample_1: TrxA, [U-95% 13C; U-95% 15N], 1.00 ± 0.05 mM; DTT 20 mM

conditions_1: ionic strength: 0.06 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1not availableconditions_1
3D CBCA(CO)NHsample_1not availableconditions_1
3D HCCH-COSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15028 7109
PDB
DBJ BAI86356 BAM54098 BAM58927 GAK79977
EMBL CAA99577 CAB14810 CBI43781 CCF06106 CCG50792
GB AAA87315 AAU24505 AAU41865 ABS74914 ADM38802
REF NP_390728 WP_003152560 WP_003184189 WP_003222500 WP_003325184
SP P14949
AlphaFold P14949

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks